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PCR 工作柜
PCC · Laminar Flow Cabinets · 5 models
By Lab Manufacturers China Technical TeamLast reviewed Standards: ISO 14644-1 (cleanroom classification), ISO 14644-3 (test methods), EN 1822 (HEPA and ULPA filters)
PCR cabinets are small enclosures for setting up polymerase chain reaction and nucleic acid work. They combine a clean or dead-air work zone with UV and often HEPA filtration to reduce contamination and cross-over between samples.
5 models of PCR Cabinets
The 5 models below are current configurations; specifications are confirmed on quotation.
静滞空气 PCR 工作站
A dead-air PCR workstation encloses reaction set-up in a still-air box with UV decontamination. It is used where physical isolation and surface decontamination matter more than filtered airflow as the main control.
垂直层流 PCR 柜
A vertical laminar flow PCR cabinet supplies HEPA-filtered air downward over the work zone with UV decontamination. It gives a clean air supply over reaction set-up as well as surface decontamination of the interior.
紫外灭菌 PCR 柜
A UV sterilizing PCR cabinet emphasises timed ultraviolet decontamination of the work zone alongside a contained front. It suits laboratories where surface decontamination between reactions is the main control for carry-over contamination.
台式 PCR 柜
A benchtop PCR cabinet is a small enclosure that fits on an existing bench for single-operator reaction set-up. It offers UV decontamination and optional HEPA flow in a compact footprint and low noise.
管道式 PCR 柜
A ducted PCR cabinet exhausts its air to atmosphere through a duct, removing airborne contamination and odour. It is chosen where recirculation is not permitted or vapours must leave the room entirely.
| Model | Type | Air supply | UV lamp | Width | Interior |
|---|---|---|---|---|---|
| Dead-Air PCR WorkstationPCC-0001 | Dead-air PCR workstation | None, still air | 254 nm, timed | 500–900 mm | Stainless or PP lined |
| Vertical Laminar Flow PCR CabinetPCC-0002 | Vertical laminar flow PCR cabinet | — | 254 nm, timed and interlocked | 600–1200 mm | Stainless steel 304 |
| UV Sterilizing PCR CabinetPCC-0003 | UV sterilizing PCR cabinet | — | — | 600–1200 mm | Reflective stainless steel |
| Benchtop PCR CabinetPCC-0004 | Benchtop PCR cabinet | Still air or HEPA downflow | 254 nm, timed | 500–900 mm | Stainless or PP lined |
| Ducted PCR CabinetPCC-0005 | Ducted PCR cabinet | — | 254 nm, timed and interlocked | 600–1200 mm | Stainless steel 304 |
Indicative values for this product class. Final specifications are confirmed on the quotation and in the calibration documents supplied with the instrument.
A PCR cabinet reduces the chance that stray DNA or RNA contaminates a reaction or that amplified product carries over into the next sample. Two mechanisms are used. A laminar flow PCR cabinet passes HEPA-filtered air across the work surface at 0.3–0.5 m/s, sweeping particles and aerosols away, and gives ISO 5 cleanliness at the work point. A dead-air or still-air workstation instead encloses the work but does not supply filtered air, relying on limited air exchange, UV irradiation and careful technique to reduce contamination. Both types usually include a 254 nm UV lamp for surface decontamination between sessions, and many include a timer and an interlock so that UV cannot run while the sash is open. Some models are ducted for vapour removal. Regular cleaning, separate pre- and post-PCR areas and dedicated equipment remain essential.
Choose a HEPA laminar flow PCR cabinet when the work needs a clean air zone over the samples, for example when preparing reactions from low-copy templates. Choose a dead-air or still-air workstation when the priority is physical isolation and UV decontamination rather than particle removal. Confirm that the UV lamp, timer and sash interlock suit the decontamination procedure, and that the internal height and footprint fit pipettes, racks and a small centrifuge. Decide whether the cabinet recirculates or is ducted, and whether a white light or blue light option is needed. For regulated diagnostics, check that the cabinet supports the workflow and cleaning validation, and verify filter performance where HEPA is fitted. Good laboratory layout with separate pre- and post-PCR zones matters as much as the cabinet itself.
PCR Cabinets are specified in PCR reaction set-up, nucleic acid extraction and handling, molecular diagnostics, forensic and research molecular work and sample preparation before amplification.
Work with PCR Cabinets is commonly governed by ISO 14644-1 (cleanroom classification), ISO 14644-3 (test methods), EN 1822 (HEPA and ULPA filters), IEST-RP-CC002 (unidirectional flow clean-air devices), ISO 15190 (medical laboratories, safety requirements) and IEC 61010-1. Instrument certifications such as CE marking are confirmed per configuration and never assumed.
Place the cabinet in the pre-PCR area for reaction set-up and keep a separate area for post-PCR work, using dedicated pipettes and consumables. Clean the work surface with a validated decontaminant before and after each session, and run the UV lamp for the set period, ensuring the sash interlock prevents exposure. Replace the UV lamp at the end of its rated life, since output falls before total failure, and check lamps with a meter if available. For HEPA models, verify filter integrity and face velocity at installation and at least annually, and change prefilters as specified. Do not use the cabinet for hazardous aerosols or chemical vapour, and keep airflow free of draughts by siting it away from doors.
It is used to set up PCR and handle nucleic acids in a small enclosed area that reduces contamination and sample cross-over. Many models add UV and HEPA filtration for cleaner working conditions.
Choose HEPA laminar flow when a clean air zone over the samples is needed, such as low-copy template work. A dead-air box suits work where physical isolation and UV decontamination matter more than particle removal.
Commonly 15–30 minutes between sessions, following the manufacturer's guidance and a validated decontamination procedure. UV is a supplement to cleaning, not a replacement, and the lamp output falls over its life.
Because amplified product is a potent contaminant and can carry over into new reactions. Separate areas, dedicated pipettes and consumables, and controlled movement reduce the chance of contaminating the pre-PCR work.
