Five-Part Differential Hematology Analyzer
五分类血液分析仪
- SKU PAR-0001
- 5-Part Analyzers
- CE (to be confirmed)
- ISO 13485 manufacturing (to be confirmed)
- NMPA class II registration (to be confirmed)
Price on request — FOB / CIF quoted per order
Prices depend on configuration, quantity and destination port.
Key specifications
| Throughput | 60–100 samples/h |
|---|---|
| Reportable parameters | 26 parameters including 5-part differential |
| Sample volume | 25 µL whole blood; 15 µL capillary mode |
| WBC linear range | 0.2–150 ×10⁹/L |
| PLT linear range | 10–1000 ×10⁹/L |
| Detection principle | Impedance plus laser flow cytometry with fluorescence |
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By Lab Manufacturers China Technical TeamLast reviewed Standards: ISO 15189, CLSI H26-A2, CLSI H20-A2
What is the Five-Part Differential Hematology Analyzer?
A five-part differential hematology analyzer is a bench-top instrument that reports a complete blood count with neutrophils, lymphocytes, monocytes, eosinophils and basophils counted separately by laser flow cytometry. It is the standard choice for hospital laboratories that act on differential results.
What is the Five-Part Differential Hematology Analyzer?
A five-part differential hematology analyzer is a bench-top instrument that reports a complete blood count with neutrophils, lymphocytes, monocytes, eosinophils and basophils counted separately by laser flow cytometry. It is the standard choice for hospital laboratories that act on differential results.
Overview
The analyzer combines impedance channels for red cells, platelets and a reference white cell count with a flow cytometry channel for the differential. Cells are stained with a fluorochrome that labels nucleic acids and membranes, then pass a laser where forward scatter, side scatter and fluorescence are recorded. Software clusters the events into five populations and generates flags for band forms, blasts, atypical lymphocytes and nucleated red cells. Typical throughput is 60 to 100 samples per hour, with 25 to 30 reportable parameters and storage of tens of thousands of records. Optional modules add reticulocyte and body fluid counts. Automatic dilution and rerun handle samples outside the linear range, and results are transmitted through HL7 or ASTM interfaces after quality control acceptance.
How does it work?
These analyzers combine impedance counting with a flow cytometry channel. A diluted and stained sample passes a sheath flow cell where hydrodynamic focusing aligns cells in single file. A laser beam strikes each cell, and three signals are recorded: forward scatter, which reflects cell size; side scatter, which reflects internal granularity and complexity; and fluorescence from a dye that stains nucleic acids and cell membranes. Clustering algorithms in the instrument software separate the signals into five leukocyte populations, and the same data support flags for band forms, blasts, atypical lymphocytes and nucleated red cells. Impedance channels measure red cells, platelets and a second white cell count for comparison. Hemoglobin is measured photometrically after lysis. Because the flow channel provides much more information than a size histogram, the analyzer can also report reticulocytes, immature platelet fraction and body fluid cell counts when the corresponding reagent and software options are installed.
What are the key features?
- Laser flow cytometry channel reporting five separate leukocyte populations
- Impedance channels for red cells, platelets and a reference white cell count
- Flags for blasts, band forms, atypical lymphocytes and nucleated red cells
- Automatic dilution and rerun for samples outside the linear range
- Three-level quality control with Levey-Jennings charts onboard
- HL7 or ASTM interface for laboratory information systems
What are the technical specifications?
A typical five-part differential hematology analyzer configuration offers throughput of 60–100 samples/h, reportable parameters of 26 parameters including 5-part differential, sample volume of 25 µL whole blood; 15 µL capillary mode, WBC linear range of 0.2–150 ×10⁹/L, PLT linear range of 10–1000 ×10⁹/L and detection principle of Impedance plus laser flow cytometry with fluorescence. Values are indicative of the product class; the exact figures for each configuration are confirmed on the quotation and the calibration documents supplied with the instrument.
Which applications is it used for?
The Five-Part Differential Hematology Analyzer is used in hospital inpatient and outpatient blood counts, chemotherapy and neutropenia monitoring, screening for hematological malignancy and pediatric blood counts. Across the wider 5-Part Analyzers class, laboratories also deploy it for hospital routine and emergency blood counts, neutropenia and chemotherapy monitoring and leukemia and lymphoma screening.
Which standards and certifications apply?
Relevant standards and methods include ISO 15189, CLSI H26-A2, CLSI H20-A2, CLSI EP05, CLSI H21, IEC 61010-1 and IEC 61010-2-101. Certification candidates for this model are CE, ISO 13485 manufacturing and NMPA class II registration; each is to be confirmed for the specific configuration before shipment, and certificates are supplied only where they have been issued.
Install the analyzer on a stable bench with a dedicated grounded outlet, controlled room temperature of about 15 to 30 degrees Celsius and low dust. Verify performance at installation with precision, carryover, linearity and method comparison studies, including a specific check of the differential against manual microscopy on abnormal samples. Run three levels of control each day, join an external quality assessment scheme, and review flag rates regularly, because an excessive flag rate increases manual reviews and delays reporting. Clean the flow cell, probes and waste lines according to the maintenance plan, protect the laser and optics from vibration, and keep sheath fluid and cleaning reagents within their expiry dates. Handle all blood samples as potentially infectious.
How do I select the right Five-Part Differential Hematology Analyzer?
Specify this variant when clinicians use differential and flag information, and when daily workload is between about one hundred and three hundred counts. In the RFQ state the required reporting parameters, whether reticulocyte and body fluid modes are needed, the minimum sample volume for pediatric work, and the interface protocol of your LIS. Ask for precision data at low white cell counts and for the sensitivity and specificity of blast flagging.
How does it compare with other 5-Part Analyzers variants?
Specify a 5-part analyzer when clinicians act on the differential, for example to follow neutropenia during chemotherapy, to screen for leukemia, or to manage pediatric infections. Compare throughput, which ranges from about 60 to more than 120 samples per hour, and the first-result time for emergency work. Check which optional modes are included: reticulocyte count, nucleated red cell count, immature platelet fraction and body fluid analysis all require dedicated reagents and software licences. Confirm the minimum sample volume for pediatric samples and the linearity at low white cell counts. Ask about the flagging performance for blasts, the review rules the software applies, and whether a slide maker or digital morphology review is supported. Confirm LIS connectivity, reagent openness, calibration interval of the flow channel, and local service support.
Full specifications
| Throughput | 60–100 samples/h |
|---|---|
| Reportable parameters | 26 parameters including 5-part differential |
| Sample volume | 25 µL whole blood; 15 µL capillary mode |
| WBC linear range | 0.2–150 ×10⁹/L |
| PLT linear range | 10–1000 ×10⁹/L |
| Detection principle | Impedance plus laser flow cytometry with fluorescence |
| Quality control | Three levels with Levey-Jennings charts |
| Data storage | 30,000 patient records |
| Interfaces | LAN with HL7 or ASTM, USB, RS-232 |
Indicative values for this product class. Final specifications are confirmed on the quotation and in the calibration documents supplied with the instrument.
Applications
- Hospital inpatient and outpatient blood counts
- Chemotherapy and neutropenia monitoring
- Screening for hematological malignancy
- Pediatric blood counts
Key features
- Laser flow cytometry channel reporting five separate leukocyte populations
- Impedance channels for red cells, platelets and a reference white cell count
- Flags for blasts, band forms, atypical lymphocytes and nucleated red cells
- Automatic dilution and rerun for samples outside the linear range
- Three-level quality control with Levey-Jennings charts onboard
- HL7 or ASTM interface for laboratory information systems
Frequently asked questions about Five-Part Differential Hematology Analyzer
The analyzer compares each cell cluster with reference populations learned during calibration and flags deviations such as additional populations in the immature region, decreased neutrophil or lymphocyte clusters, or an abnormal eosinophil position. Flagging rules are defined by the manufacturer and should be verified locally against manual microscopy.
A 5-part analyzer counts and classifies cells from their optical signals and reports numeric results with flags. A digital morphology system prepares a stained film, scans it, and presents images to a technologist for review, so it provides cell morphology rather than a count. Many laboratories use both.
Yes, when the body fluid mode and its reagents are installed. It reports total cells, red cells and white cells, and in some models differentiates mononuclear and polymorphonuclear cells in cerebrospinal, pleural, peritoneal and synovial fluid. The mode must be validated against manual chamber counts locally.
A high flag rate usually reflects a patient population with many abnormal samples, incorrect reference limits, or software rules set too sensitively. Laboratories should review the flag list with the supplier, adjust only thresholds they can justify, and accept that a certain rate of manual review is necessary for patient safety.
A 5-part differential reports neutrophils, lymphocytes, monocytes, eosinophils and basophils separately rather than as grouped populations. This supports monitoring of neutropenia, allergic and parasitic conditions, and abnormal white cell patterns, and the underlying flow cytometry data also allow flags for immature cell populations.
No. Analyzers flag many abnormalities, but morphological assessment of blasts, dysplastic cells, malaria parasites and red cell shape still needs a stained film and microscopy. Most laboratories review films for a defined proportion of samples or whenever specific flags appear, as part of their validation and review rules.
Reticulocyte and nucleated red cell counts are useful for anemia assessment, the immature platelet fraction helps distinguish platelet destruction from reduced production, and body fluid modes support cerebrospinal and synovial fluid cell counts. Each module needs dedicated reagent and software, so order only what the clinical service will use.
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