Multi-Angle Dynamic Light Scattering Nanoparticle Analyzer

What is the Multi-Angle Dynamic Light Scattering Nanoparticle Analyzer?

A dynamic light scattering system measuring at several scattering angles, typically 15°, 90° and 173°, to resolve polydisperse or multimodal nanoparticle samples more reliably than single-angle DLS. It provides intensity, volume and number distributions and helps detect small fractions of large aggregates.

Overview

Multi-angle DLS collects correlation functions at forward, side and backscatter angles and combines them into a single, more robust size distribution. Forward angles are sensitive to large particles and aggregates, while backscatter tolerates turbid and concentrated samples. Because different angles weight size populations differently, the analysis can separate modes that overlap at a single angle and check that results are angle-independent, a sign of a valid sample. A high-power 633 nm laser with avalanche photodiodes and motorised goniometer or fixed multi-detector arrangement is used. Temperature control spans 0 to 90 °C, and a range of 0.3 nm to 10 µm is typical. The system gives intensity-weighted sizes; converting to volume or number depends on optical models, so such results should be regarded as estimates.

How does it work?

Nanoparticle tracking analysis illuminates a dilute suspension with a laser sheet and records the scattered light of individual particles with a camera; software follows each track and derives a diffusion coefficient, converted to hydrodynamic diameter with the Stokes–Einstein equation, and counts particles per millilitre in a known volume. Resistive pulse sensing passes particles through a nanopore in an electrolyte; each particle transiently reduces ionic current in proportion to its volume, giving size and concentration. Field-flow fractionation separates particles in a thin channel by diffusion under a cross-flow before in-line light scattering detectors measure size and molar mass. Nano flow cytometers use hydrodynamic focusing and sensitive side-scatter and fluorescence detection to phenotype single particles. Sample dilution and calibration with reference beads are essential.

What are the key features?

• Forward, side and backscatter detection angles in one system
• Improved resolution of overlapping populations
• Angle-independence check for sample validity
• Backscatter option for turbid and concentrated samples
• Peltier cell holder from 0 to 90 °C
• Optional automated titration or plate reader accessory

What are the technical specifications?

A typical multi-angle dynamic light scattering nanoparticle analyzer configuration offers size range of 0.3 nm–10 µm, detection angles of 15°, 90°, 173°, laser of 633 nm, ≥ 10 mW, detector of Avalanche photodiode, correlator of Digital, ≥ 1000 channels, ≥ 10 ns and sample volume of ≥ 3 µL (low-volume cell). Values are indicative of the product class; the exact figures for each configuration are confirmed on the quotation and the calibration documents supplied with the instrument.

Which applications is it used for?

The Multi-Angle Dynamic Light Scattering Nanoparticle Analyzer is used in multimodal lipid and polymer nanoparticles, detection of aggregates in protein solutions, concentrated or turbid nanoparticle samples, stability studies versus temperature and quality control of nanoparticle batches. Across the wider Nanoparticle Analyzers class, laboratories also deploy it for liposome and lipid nanoparticle characterisation, extracellular vesicle and exosome research and viral vector and vaccine particle counting.

Which standards and certifications apply?

Relevant standards and methods include ISO 19430, ISO 13322-1, ISO 22412, ISO/TS 21362, ASTM E2834, ISO 21363 and GB/T 19627. Certification candidates for this model are CE and ISO 9001 manufacturing; each is to be confirmed for the specific configuration before shipment, and certificates are supplied only where they have been issued.

Nanoparticle measurements are very sensitive to sample preparation. Use particle-free, filtered diluents and clean glassware, and dilute to the instrument's optimum concentration window. Dust and bubbles produce false counts. Verify size and concentration with certified polystyrene or silica nanosphere standards at start-up and after changes of settings, and report camera level, detection threshold, temperature and number of tracks, because these parameters change results. Keep laser optics, flow cells and nanopores clean and stored as advised. Install in a stable, temperature-controlled room. Regulated work needs IQ/OQ/PQ, locked methods and audit trails.

How do I select the right Multi-Angle Dynamic Light Scattering Nanoparticle Analyzer?

Choose multi-angle DLS for polydisperse or multimodal suspensions below 10 µm where single-angle results are ambiguous and concentration data are not required. Specify angles, concentration range, sample volume, temperature control and any accessory needs. If particle concentration or single-particle subpopulations matter, choose tracking or cytometric variants instead.

How does it compare with other Nanoparticle Analyzers variants?

Decide what the measurement must deliver: concentration and size distribution of polydisperse samples, fluorescent subpopulation counts, high-resolution separation of mixtures, or single-particle phenotype. Nanoparticle tracking suits 30 to 1000 nm particles at 10⁷ to 10⁹ particles/mL. Resistive pulse sensing gives high-resolution, refractive-index-independent size for conductive dispersions. Field-flow fractionation coupled to multi-angle light scattering resolves mixtures and aggregates. Nano flow cytometry is preferred when labelling and marker analysis are needed. Check detection limit versus your smallest particle, required dynamic range, sample volume, and optical property dependence. Consider whether applicable guidance such as ISO 19430 and regulatory expectations for liposomes or vaccines apply, and who will operate and maintain the system.